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KMID : 0545120080180101678
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 10 p.1678 ~ p.1682
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Diversity Analysis of Lactic Acid Bacteria in Takju, Korean Rice Wine
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Jin Jianbo
Eom Hyun-Ju
Han Nam-Soo
Kim So-Young
Jin Qing
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Abstract
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To investigate the lactic acid bacterial population in Korean traditional rice wines, biotyping was performed using cell morphology and whole-cell protein pattern analysis by SDSPAGE, and then the isolates were identified by 16S rRNA sequencing analysis. Based on the morphological characteristics, 103 LAB isolates were detected in wine samples, characterized by whole-cell protein pattern analysis, and they were then divided into 18 patterns. By gene sequencing of 16S rRNA, the isolates were identified as Lactobacillus paracasei, Lb. arizonensis, Lb. plantarum, Lb. harbinensis, Lb. parabuchneri, Lb. brevis, and Lb. hilgardii when listed by their frequency of occurrence. It was found that the difference in bacterial diversity between rice and grape wines depends on the raw materials, especially the composition of starch and glucose.
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KEYWORD
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Takju, lactic acid bacteria, SDS-PAGE, 16S rRNA
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